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Postsynaptic Recordings at Afferent Dendrites Contacting Cochlear Inner Hair Cells: Monitoring Multivesicular Release at a Ribbon Synapse
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Postsynaptic Recordings at Afferent Dendrites Contacting Cochlear Inner Hair Cells: Monitoring Multivesicular Release at a Ribbon Synapse

Postsynaptic Recordings at Afferent Dendrites Contacting Cochlear Inner Hair Cells: Monitoring Multivesicular Release at a Ribbon Synapse

DOI:
10.3791/2442-v

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11:45 min

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February 10, 2011

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, , ,
1Department of Otolaryngology-Head and Neck Surgery.,The Johns Hopkins School of Medicine, 2Instituto de Investigaciones en Ingeniería Genética y Biología Molecular,Consejo Nacional de Investigaciones Científicas y Técnicas

Chapters

  • 00:05Title
  • 01:08Prepare Tissue Holders and Electrodes
  • 01:57Dissecting the Cochlear Tissue Sample
  • 05:03Placing Electrodes for Inner Hair Cells and Afferent Boutons
  • 08:43Results: Recordings from Afferent Fibers and Inner Hair Cells
  • 11:26Conclusion

Summary

Automatic Translation

Automatic Translation

Whole-cell patch-clamp recordings from auditory nerve fiber dendrites at the inner hair cell ribbon synapse in the mammalian cochlea.

Tags

Postsynaptic RecordingsAfferent DendritesCochlear Inner Hair CellsMultivesicular ReleaseRibbon SynapseAuditory Nerve FiberIHC Membrane PotentialExcitatory Postsynaptic Currents (EPSCs)Presynaptic RibbonAuditory Nerve FibersUnmyelinated Single EndingAfferent BoutonsTemporal ResolutionPre- And Postsynaptic CellsNeurotransmission

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