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Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney
 

Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney

Article doi: 10.3791/2555
April 22nd, 2011

Summary April 22nd, 2011

In this report we describe a method for the isolation and culture of the progenitor cell niche from the embryonic mouse kidney that can be used to study signaling pathways regulating stem/progenitor cells of the developing kidney. These cultured cells are highly accessible to small molecule and recombinant protein treatment, and importantly also to viral transduction, which allows efficient manipulation of candidate pathways.

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