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April 22, 2011
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In this report we describe a method for the isolation and culture of the progenitor cell niche from the embryonic mouse kidney that can be used to study signaling pathways regulating stem/progenitor cells of the developing kidney. These cultured cells are highly accessible to small molecule and recombinant protein treatment, and importantly also to viral transduction, which allows efficient manipulation of candidate pathways.
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Cite this Article
Brown, A. C., Blank, U., Adams, D. C., Karolak, M. J., Fetting, J. L., Hill, B. L., Oxburgh, L. Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney. J. Vis. Exp. (50), e2555, doi:10.3791/2555 (2011).
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