Journal
/
Biology
/
Microarray Analysis for Saccharomyces cerevisiae
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Microarray Analysis for Saccharomyces cerevisiae

Microarray Analysis for Saccharomyces cerevisiae

DOI:
10.3791/2585-v

•

13:17 min

•

April 07, 2011

•

, , ,
1Vermont Genetics Network,The University of Vermont

Chapters

  • 00:05Title
  • 02:18Extracting Total RNA from Yeast Cultures
  • 05:03Microarray Target Preparation
  • 09:44Results of Microarray Analysis
  • 10:56Conclusion

Summary

Automatic Translation

Automatic Translation

In this protocol, gene expression in yeast (Saccharomyces cerevisiae) is changed after exposure to oxidative stress induced by the addition of hydrogen peroxide (H2O2), an oxidizing agent.

Tags

Microarray AnalysisSaccharomyces CerevisiaeGene ExpressionOxidative StressHydrogen PeroxideYeast CultureGlucoseControl GroupTreated GroupHanks Buffered SalineTotal RNA ExtractionBiotin-labeled CRNAUVM Microarray Core FacilityAffymetrix Yeast GeneChipsGene Expression Data Analysis

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/2585?article_type=v

Dissection of Saccharomyces Cerevisiae Asci
check_url/2585?article_type=v

Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae
check_url/2585?article_type=v

A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae
check_url/2585?article_type=v

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
check_url/2585?article_type=v

Coupled Assays for Monitoring Protein Refolding in Saccharomyces cerevisiae
check_url/2585?article_type=v

EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1
check_url/2585?article_type=v

Rapid Identification of Chemical Genetic Interactions in Saccharomyces cerevisiae
check_url/2585?article_type=v

Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae
check_url/2585?article_type=v

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
check_url/2585?article_type=v

Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae

Read Article