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En metod för äggblåsa Inkapsling och kultur i ett Proteolytically Nedbrytbara 3 dimensionellt system
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1Institute for BioNanotechnology in Advanced Medicine,Northwestern University, 2Department of Obstetrics and Gynecology,Northwestern University, Feinberg School of Medicine, 3Center for Reproductive Research,Northwestern University, 4The Robert H. Lurie Comprehensive Cancer Center,Northwestern University, 5Department of Chemical and Biological Engineering,Northwestern University
Chapters
- 00:05Title
- 03:02Follicle Isolation
- 05:11The Drop Method
- 07:16The Parafilm Method
- 08:31Follicle Recovery from the 3D Matrix
- 10:00Alginate and Fibrin-alginate for 3D Follicle Culture in Vitro
- 11:48Conclusion
En ny metod för äggblåsa inkapsling i en 3D fibrin-alginat genomträngande nätverk beskrivs. Detta system kombinerar strukturellt stöd med proteolytisk nedbrytning för att stödja utvecklingen av omogna folliklar att producera mogna ägg. Denna metod kan tillämpas på aggregaten kultur cellen att upprätthålla cell-cell kontakter utan att begränsa expansionen.
Tags
Ovarian FollicleEncapsulationCultureProteolytically Degradable3 Dimensional SystemIn Vitro Follicle TechniquesHydrogelsNative Ovarian Environment3D Follicular ArchitectureCell-cell InteractionsParacrine SignalingAlginateInert Algae-derived PolysaccharideGelationCalcium IonsDevelopmental CompetenceDegradableFibrin-alginate Interpenetrating Network (FA-IPN)Dynamic Mechanical Environment
