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JoVE Journal
Immunology and Infection

This content is Open Access.

Quantitative Assessment of Immune Cells in the Injured Spinal Cord Tissue by Flow Cytometry
 

Quantitative Assessment of Immune Cells in the Injured Spinal Cord Tissue by Flow Cytometry: a Novel Use for a Cell Purification Method

Article DOI: 10.3791/2698
April 9th, 2011

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Summary April 9th, 2011

Quantification of cellular inflammation in the injured/pathological CNS by flow cytometry is complicated by lipid/myelin debris that can have similar size and granulation to cells, decreasing sensitivity/accuracy. We have advanced a cell preparation method to remove myelin debris and improve cell detection by flow cytometry in the injured spinal cord.

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