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β细胞死亡介导细胞毒性T淋巴细胞的方法来评估
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Methods to Assess Beta Cell Death Mediated by Cytotoxic T Lymphocytes
DOI:

12:12 min

June 16, 2011

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Chapters

  • 00:05Title
  • 01:38Introduction
  • 02:29Cell Culture
  • 04:42Cell-mediated Lymphocytotoxicity (CML Assay)
  • 07:29Live Cell Imaging to Asses CTL-mediated Beta Cell Death
  • 10:10Representative Cell Death Assay Data
  • 11:39Conclusion

Summary

Automatic Translation

细胞介导的​​淋巴细胞毒(CML)的检测,可用于测试自身反应性的反应,并在体外研究细胞死亡的机制。然而,利用活细胞共聚焦显微成像技术与荧光染料,类型和细胞死亡以及途径利用动力学可以更详细的研究。

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