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JoVE Journal
Immunology and Infection

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Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation
 

Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation

Article doi: 10.3791/2766
July 8th, 2011

Summary July 8th, 2011

We present our optimized high-throughput nucleofection protocol as an efficient way of transfecting primary human monocyte-derived dendritic cells with either plasmid DNA or siRNA without causing cell maturation. We further provide evidence for successful siRNA silencing of targeted gene RIG-I at both the mRNA and protein levels.

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