Rig-i Silencing
Rig-i Silencing - Related Videos
08:47
Gene Silencing with Morpholinos
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2023
Morpholino-mediated gene silencing is a common technique used to study roles of specific genes during development. Morpholinos inhibit gene expression by hybridizing to complementary mRNAs. Due to their unique chemistry, morpholinos are easy to produce and store, which makes them remarkably cost effective compared to other gene silencing methods. This video reviews proper experimental design when using these oligonucleotides. Following that, an explanation of morpholino microinjection...
01:26
Chemogenetic Silencing of Neuronal Activity in a Neonatal Mouse
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2025
Place an anesthetized neonatal pup on a heating pad.Disinfect the scalp with ethanol.Fill a syringe with a viral solution containing adeno-associated viruses carrying the gene for a drug-activated inhibitory receptor and a tracking dye.Align the syringe tip with the pup’s head and slowly inject the virus into the brain for even distribution.Allow the pup to recover.During incubation, viral particles enter neurons and release their genetic material into the nucleus.It leads to inhibitory...
12:43
Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE
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Cited by 12 •
2014
Innate defenses to virus infections are triggered by pattern recognition receptors (PRRs). The two cytoplasmic PRRs RIG-I and PKR bind to viral signature RNAs, change conformation, oligomerize, and activate antiviral signaling. Methods are described which allow to conveniently monitor the conformational switching and the oligomerization of these cytoplasmic PRRs.
06:34
Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato
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Cited by 159 •
2009
Description of a virus-induced gene silencing (VIGS) method for knock-down of gene expression in Nicotiana benthamiana and tomato.
10:18
Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
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Cited by 159 •
2011
We present the detailed protocol for Agrobacterium-mediated virus-induced gene silencing (VIGS) assay in cotton. The tobacco rattle virus (TRV)-derived VIGS vectors were deployed to induce RNA silencing of cotton GrCLA1, Cloroplastos alterados 1 gene. The albino phenotype caused by silencing GrCLA1 was observed at the seedling stage within 2 weeks after inoculation.
FAQs
Reducing RIG-I expression or activity lowers the sensor input available to MAVS, the signaling adaptor downstream of RIG-I. This can weaken activation of interferon and inflammatory gene expression after viral RNA recognition. Comparing these responses with those in cells retaining RIG-I helps researchers determine how strongly this sensor contributes to a particular antiviral response.
These viral RNA features provide the recognition context in which RIG-I normally contributes to innate immune signaling. When the sensor is silenced, changes in responses to RNA containing such features can reveal whether those effects depend on RIG-I. This approach connects a defined viral RNA signal to downstream interferon and inflammatory outcomes.
RNA interference reduces RIG-I expression through an RNA-directed mechanism, whereas transcriptional repression limits production of RIG-I at the gene-expression stage. Both approaches decrease signaling capacity, but they intervene at different points. Using either strategy allows investigators to examine the consequences of reduced sensor availability while keeping the experimental focus on RIG-I-dependent antiviral signaling.
The strategy separates responses associated with RIG-I from broader cellular reactions to infection. If interferon or inflammatory gene expression changes after RIG-I reduction, the result supports a contribution from this sensor pathway, including its connection to MAVS. Such comparisons help clarify whether a viral response depends on RIG-I recognition or reflects other host immune mechanisms.
A study first applies a supported silencing strategy, such as RNA interference or transcriptional repression, to reduce RIG-I expression or activity. Researchers then examine host responses during viral exposure, focusing on interferon and inflammatory gene expression. Interpreting the altered response alongside the silenced condition helps connect infection-related outcomes to RIG-I pathway function.
It is useful when researchers need to test whether a pathogen alters or bypasses recognition through the RIG-I pathway. Reducing the sensor provides a perturbation for examining how viral RNA features, MAVS signaling, and downstream interferon responses relate to infection. These experiments can clarify how host defenses operate and how pathogens manipulate innate immune signaling.