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Vibratome切片增强保尔蒂哺乳动物器官的细胞构筑
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Neuroscience
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JoVE Journal Neuroscience
Vibratome Sectioning for Enhanced Preservation of the Cytoarchitecture of the Mammalian Organ of Corti
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Vibratome切片增强保尔蒂哺乳动物器官的细胞构筑

DOI:
10.3791/2793-v

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05:58 min

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June 17, 2011

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1Department of Pediatrics, Children’s Research Institute,Medical College of Wisconsin

Chapters

  • 00:05Tile
  • 01:22Dissection and Fixation of Inner Ears
  • 04:07Mounting the Sections for Confocal Microscopy
  • 04:53Antibody Staining and Confocal Microscopy of Pillar Cells in the Organ of Corti
  • 05:28Conclusion

Summary

Automatic Translation

Automatic Translation

一个简单的程序，切片，免疫组织化学和激光共聚焦显微镜Corti器的vibratome描述。此过程允许的罚款尔蒂哺乳动物器官的细胞结构的改善保存，因此可以准确定量的细胞类型。

Tags

Vibratome SectioningCytoarchitectureMammalian Organ Of CortiCellular MosaicMechanosensory Hair CellsNonsensory Supporting CellsCross-sectioningHistological ProceduresParaffin SectioningCryo-sectioningImmunohistochemical StainingVibratome SectionsConfocal MicroscopyImmunohistochemical AnalysisMutant Organs Of Corti

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