Immunology and Infection
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Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins
Chapters
Summary July 1st, 2011
An efficient method to assess surface-exposure of leptospiral proteins is described. The method is specifically designed to avoid disruption of the fragile outer membrane of leptospiral cells. This technique requires employment of several negative controls to assess the integrity of the outer membrane and specificity of antibody reaction.
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