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Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins

Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins

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10:47 min

July 01, 2011

DOI:

10:47 min
July 01, 2011

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Summary

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An efficient method to assess surface-exposure of leptospiral proteins is described. The method is specifically designed to avoid disruption of the fragile outer membrane of leptospiral cells. This technique requires employment of several negative controls to assess the integrity of the outer membrane and specificity of antibody reaction.

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