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小鼠神经干细胞移植手术与器官的诱向性鼠肝炎病毒感染小鼠的脊髓
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Surgical Transplantation of Mouse Neural Stem Cells into the Spinal Cords of Mice Infected with Neurotropic Mouse Hepatitis Virus
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小鼠神经干细胞移植手术与器官的诱向性鼠肝炎病毒感染小鼠的脊髓

DOI:
10.3791/2834-v

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08:33 min

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July 10, 2011

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, , , ,
1Department of Molecular Biology and Biochemistry,University of California, Irvine, 2Sue and Bill Gross Stem Cell Center,University of California, Irvine, 3Institute for Immunology,University of California, Irvine

Chapters

  • 00:05Title
  • 01:25Preparation for Surgery and Cells for Transplant
  • 02:54Surgery and Transplantation
  • 04:45Injection of Cells
  • 07:00Sutures and Post-op Care
  • 07:53Successful Spinal Cord Transplantation
  • 08:07Conclusion

Summary

Automatic Translation

Automatic Translation

小鼠神经干细胞分化到小鼠建立脱髓鞘脊髓移植是详细的。神经干细胞的准备工作，椎板切除胸椎9（T9），神经干细胞移植概述随着小鼠前和术后护理。

Tags

Surgical TransplantationMouse Neural Stem CellsSpinal CordsMice InfectedNeurotropic Mouse Hepatitis VirusJHM StrainDemyelinating DiseaseMultiple SclerosisTransplantation Of NSCsRemyelinationClinical OutcomeCell Replacement TherapiesChronic Neurologic DiseasesIn Vivo ModelsEngrafted CellsAdapted MethodI) Preparation Of NSCsIi) Pre-operative Care Of MiceIii) Exposure Of The Spinal Cord Via LaminectomyIv) Stereotactic Injection Of NSCsIv) Post-operative Care

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