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条形码酵母图书馆竞争力的基因屏幕
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Competitive Genomic Screens of Barcoded Yeast Libraries
DOI:

11:59 min

August 11, 2011

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Chapters

  • 00:05Title
  • 01:55Combining Individual Mutants into a Single Pool
  • 03:23Experimental Pool Growth
  • 04:56Genomic DNA Extraction, PCR and Microarray Hybridization
  • 08:33Sequencing Barcoded Yeast Strains
  • 09:22Genomic Screening Results
  • 11:03Conclusion

Summary

Automatic Translation

我们已经开发出全面的,无偏见的全基因组的屏幕,了解基因药物和基因与环境的相互作用。筛选这些突变集合的方法。

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