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JoVE Journal
Immunology and Infection

This content is Open Access.

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
 

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

Article DOI: 10.3791/2953
June 16th, 2011

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Summary June 16th, 2011

The present article describes the steps required to isolate and characterize RNA polymerase fidelity variants of RNA viruses and how to use mutation frequency data to confirm fidelity changes in tissue culture.

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