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JoVE Journal
Neuroscience

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Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation
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Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation

Article DOI: 10.3791/2957
June 23rd, 2011 •
1, 2, 1, 1,2, 1, 2, 1
1Department of Biochemistry and Molecular Biology, Hotchkiss Brain Institute, Alberta Children’s Hospital Research Institute, University of Calgary, 2Department of Medical Genetics, Alberta Children’s Hospital Research Institute, Hotchkiss Brain Institute, University of Calgary

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Summary
Automatic Translation
June 23rd, 2011

In utero electroporation allows for rapid gene delivery in a spatially- and temporally-controlled manner in the developing central nervous system (CNS). Here we describe a highly adaptable in utero electroporation protocol that can be used to deliver expression constructs into multiple embryonic CNS domains, including the telencephalon, diencephalon and retina.

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