Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Targeting Olfactory Bulb Neurons Using Combined…
JoVE Journal
Neuroscience

This content is Open Access.

JoVE Journal Neuroscience
Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines
Hippocampal Insulin Microinjection and In vivo Microdialysis During Spatial Memory Testing
Journal (Neuroscience)
Hippocampal Insulin Microinjection and In vivo Microdialysis During Spatial Memory Testing
Visualization of Larval Segmental Nerves in 3rd Instar Drosophila Larval Preparations
Journal (Neuroscience)
Visualization of Larval Segmental Nerves in 3rd Instar Drosophila Larval Preparations
 

Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines

Article DOI: 10.3791/2964-v • 08:18 min • August 15th, 2011
August 15th, 2011 •
1, 2, 3, 1
1Department of Biology, Pace University, 2Cellular and Molecular Medicine, University of California, San Diego, 3Division of Cell Biology and Cell Physiology, Zoological Institute, Braunschweig University of Technology

Chapters

Summary
Automatic Translation

The temporal and spatial resolution of genetic manipulations determines the spectrum of biological phenomena that they can perturb. Here we use temporally and spatially discrete in vivo electroporation, combined with transgenic lines of zebrafish, to induce expression of a GFP transgene specifically in neurons of the developing olfactory bulb.

Tags

Olfactory Bulb Neurons In Vivo Electroporation Gal4-based Enhancer Trap Zebrafish Lines DNA Expression Plasmids RNAi Reagents Morpholino Anti-sense Oligonucleotides Developing Embryos C. Elegans Chick Xenopus Mouse Spatial Resolution Temporal Resolution Transgenic Approaches Specific Cell Population Developing Nervous System
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter