Developing a Translaminar Pressure Model Using a Translaminar Autonomous System

Begin with a human eye. Remove the sheath to expose the optic nerve.

Bisect the eye, then remove the vitreous humor from the posterior segment.

Trim the sclera, the outer layer, and spread the retina containing the retinal ganglion cells connected to the optic nerve via the lamina cribrosa, a mesh-like structure.

Place this segment over the dome of the lower chamber of the translaminar autonomous system, with the optic nerve facing upwards then seal it.

Connect the tubing and flow a perfusion medium.

The liquid's movement builds pressure inside the eye cup, resembling intraocular pressure.

Next, position the upper chamber covering the optic nerve and seal it.

Connect the tubing to flow the medium around the nerve, resembling intracranial pressure.

The pressure difference between these chambers establishes a translaminar pressure across the lamina cribrosa, enabling the study of retinal ganglion cell signal transmission to the optic nerve under this pressure condition.

To prepare a human whole eye globe sample, remove the optic nerve sheath and remove the vitreous humor from the posterior segment. To ensure a good fit on the round dome of the IOP chamber, trim additional sclera from the pos