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Expansion of Human Peripheral Blood γδ T Cells using Zoledronate
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Immunology and Infection
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JoVE Journal Immunology and Infection
Expansion of Human Peripheral Blood γδ T Cells using Zoledronate

Expansion of Human Peripheral Blood γδ T Cells using Zoledronate

DOI:
10.3791/3182-v

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13:08 min

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September 09, 2011

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, , , , , ,
1Department of Immunotherapeutics (Medinet),University of Tokyo Hospital, 2MEDINET Co., Ltd

Chapters

  • 00:05Title
  • 01:17Isolation of Peripheral Blood Mononuclear Cells (PBMC)
  • 03:16Expansion of γδ T Cells
  • 05:55Phenotypic Analysis by Flow Cytometry
  • 06:57IFN-gamma Production Assay
  • 09:40Representative Results
  • 12:33Conclusion

Summary

Automatic Translation

Automatic Translation

A method to expand γδ T cells from peripheral blood mononuclear cells (PBMC) is described. PBMC-derived γδ T cells are stimulated and expanded using zoledronate and interleukin-2 (IL-2). Large scale expansion of γδ T cells can be applied to autologous cellular immunotherapy of cancer.

Tags

Human Peripheral Bloodγδ T CellsExpansionZoledronateStress-induced AntigensAnti-tumor ActivityAnti-infective ActivityVγ9Vδ2 T Cell ReceptorMajor Histocompatibility Complex-independentCytolytic FunctionsTh1-like Effector FunctionsCancer ImmunotherapyPhosphoantigens(E)-4-hydroxy-3-methyl-but-2-enyl Pyrophosphate (HMBPP)Isoprenoid BiosynthesisIsopentenyl Pyrophosphate (IPP)Mevalonate PathwayDysregulationTumor CellsAminobisphosphonatesPamidronateFarnesyl Pyrophosphate Synthase (FPPS)Intracellular Levels Of IPP

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