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Eksperimentell Generation of Karsinom-Associated Fibroblaster (kafeer) fra Human mammary Fibroblaster

Article DOI: 10.3791/3201-v • 15:43 min • October 25th, 2011
October 25th, 2011 •
*1, *1, 1,2
1CR-UK Stromal-Tumour Interaction Group, Paterson Institute for Cancer Research, University of Manchester, 2Atopy Research Center, Juntendo University
* These authors contributed equally

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Karsinom-assosiert fibroblaster (kafeer) rik på myofibroblasts stede i tumor stroma, spille en viktig rolle i å drive tumor progresjon. Vi utviklet en coimplantation svulst xengraft modell for eksperimentelt generere kafeer fra menneskelige mammary fibroblaster. Protokollen beskriver hvordan å etablere CAF myofibroblasts at få en evne til å fremme tumourigenesis.

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Medisin kreft stromal myofibroblasts eksperimentelt genererte carcinoma-assosiert fibroblaster (exp-kafeer) fibroblast menneskelige mammary karsinom tumor xenografts
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