Breast Carcinoma Cells

Breast carcinoma cells are abnormal epithelial cells originating in breast tissue that acquire uncontrolled growth and can form malignant tumors, making them central to breast cancer diagnosis and research. Genetic and epigenetic alterations disrupt normal controls on cell division, survival, adhesion, and movement, allowing these cells to proliferate, invade surrounding tissue, and sometimes spread to distant organs. Researchers examine breast carcinoma cells in patient samples and laboratory models to identify biomarkers, classify tumor subtypes, and evaluate drug responses. Studying their behavior supports earlier detection, more precise prognostic assessment, and development of targeted therapies for breast cancer.

Breast Carcinoma Cells - Related Videos

Research

JoVE Journal - Medicine
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Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer

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Cited by 27 •

2014

Activation of latent mutations with adenovirus-Cre into the mammary ductal system results in a clinically relevant metastatic breast cancer. Incorporation of a YFP promoter allows tracking of distal metastatic tumor cells. This model is useful to study latent metastasis, anti-tumor immunity, and for designing novel immunotherapies to treat breast cancer.

Research

JoVE Journal - Bioengineering

Preparation and Analysis of In Vitro Three Dimensional Breast Carcinoma Surrogates

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Cited by 6 •

2016

We demonstrate a method to generate 3D breast cancer surrogates, which can be cultured using a perfusion bioreactor system to deliver oxygen and nutrients. Following growth, surrogates are fixed and processed to paraffin for evaluation of parameters of interest. The evaluation of one such parameter, cell density, is explained.

Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts

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Cited by 25 •

2011

Carcinoma-associated fibroblasts (CAFs) rich in myofibroblasts present within the tumour stroma, play a major role in driving tumour progression. We developed a coimplantation tumour xengraft model for experimentally generating CAFs from human mammary fibroblasts. The protocol describes how to establish CAF myofibroblasts that acquire an ability to promote tumourigenesis.

Modeling Spontaneous Metastatic Renal Cell Carcinoma (mRCC) in Mice Following Nephrectomy

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Cited by 23 •

2014

Models of spontaneous metastatic renal cell carcinoma (RCC) disease progression can be used for evaluating treatments in a clinically relevant setting. This protocol demonstrates different procedures for orthotopic kidney tumor cell implantation, proper nephrectomy, and finally outlines a necropsy guide for visual and bioluminescent scoring of metastatic burden and localization.

Evaluation of Stem Cell Properties in Human Ovarian Carcinoma Cells Using Multi and Single Cell-based Spheres Assays

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Cited by 8 •

2015

In vitro spheres assays are commonly used to identify cancer stem cells. Here we compare single with multi cell-based spheres assays. The more laborious single cell-based assays or methylcellulose supplementation give more accurate results while multi cell-based assays performed in liquid medium can be highly influenced by cell density.

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