Transplantation of Human-Derived Interneuron Precursor Cells into the Mouse Pup Hippocampus

Secure an anesthetized genetically modified mouse pup in a stereotaxic frame using opposite-direction ear bars to prevent injury.

The pup’s hippocampus has reduced inhibitory interneurons, leading to hyperexcitation of excitatory neurons.

Maintain a flat head position and cover the pup with ice to prolong anesthesia.

Disinfect the skull and identify the lambda suture as a reference point.

Position the injection needle at the target hippocampal coordinates.

Using a bent needle, create a microentry hole while minimizing skull damage.

Lower the needle to the target depth and inject human-derived interneuron precursor cells at a controlled rate, minimizing tissue damage.

Slowly retract the needle to prevent cell reflux.

Remove the pup from the frame, warm it until movement resumes, then return it to the litter in the cage.

The injected precursor cells disperse across the hippocampus, mature, and integrate into the neuronal network. Over time, they enhance inhibitory transmission, which restores excitatory-inhibitory balance.

Position the pup by placing it on the stereotaxic frame, and using the ear bars in the opposite direction. Next, clean the surface of the skin using a soft tissue soaked in