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Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

DOI:
10.3791/3273-v

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10:47 min

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October 28, 2011

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, , , , ,
1San Diego Regenerative Medicine Institute, 2Xcelthera, 3Department of Neurosurgery,Harvard Medical School, 4Division of SCI Research, VA Boston Healthcare System, 5Program in Stem Cell & Regenerative Biology,Sanford-Burnham Medical Research Institute, 6La Jolla IVF

Chapters

  • 00:05Title
  • 01:27Media Preparation and Plate Coating
  • 02:33Passaging and Seeding Undifferentiated Human ES Cells
  • 04:31Neural Induction in Adhesive Culture with Retinoic Acid
  • 05:42Differentiation in Suspension Culture and Maturation to Neuronal Phenotype after Attachment
  • 08:08Direct Neuronal Differentiation of Feeder- and Serum- Free Pluripotent Human ES Cells WIth Retinoic Acid Induction
  • 10:11Conclusion

Summary

Automatic Translation

Automatic Translation

We have established a protocol for induction of neuroblasts direct from pluripotent human embryonic stem cells maintained under defined conditions with small molecules, which enables derivation of a large supply of human neuronal progenitors and neuronal cell types in the developing CNS for neural repair.

Tags

Human Neuronal ProgenitorsNeuronsPluripotent Human Embryonic Stem CellsSmall Molecule InductionCell-based TherapiesCentral Nervous System (CNS)Neural Stem CellsExpansion AbilityPlasticityTrophic And Neuroprotective MoleculesPluripotent HESCsNeurological DisordersDifferentiation PotentialDevelopmental StudyClinical TranslationMulti-lineage InclinationGerm Layer

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