Intravenous Administration of Recombinant Adeno-Associated Virus Vectors in a Rat Model

Begin with an anesthetized rat positioned on its side with its tail exposed.

Locate the lateral tail vein, identify an injection site along its length, and disinfect the area.

Apply slight pressure above the injection site to dilate the vein.

Take a syringe containing the recombinant adeno-associated virus or AAV vector carrying the target gene.

Align the needle with the vein and pierce the tail skin.

Release the pressure on the vein to restore normal blood flow.

Insert the needle into the tail vein and slowly inject the virus.

After injection, withdraw the needle, apply pressure to stop any bleeding, and monitor the rat's recovery.

The lateral tail vein delivers the virus into the bloodstream, where it circulates through the heart and enters the brain, reaching the neurons.

Inside the neuron, the virus enters the nucleus, releases its genetic material, and facilitates the production of target proteins for neurotherapeutic applications.

Properly anesthetize the rat using the anesthesia system. Ensure an adequate plane of anesthesia by the absence of a reaction to a toe pinch. Then, place the rat in the work area on its side and maintain anesthesia using the nose cone. With the rat lying on