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旋转的人类细胞培养的细胞培养系统:作为一种模式的滋养细胞
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model
DOI:

06:54 min

January 18, 2012

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Chapters

  • 00:05Title
  • 01:04EVT Cells and Collagen Bead Incubation
  • 02:13Loading the Rotating Cell Culture System (RCCS)
  • 03:41Changing the Media
  • 04:37Collecting Propagated Cells
  • 05:39SGHPL-4 Trophoblast Cells in a 3D Milieu
  • 06:30Conclusion

Summary

Automatic Translation

传统的,二维细胞培养技术,往往导致与分化标志物,细胞因子和生长因子的改变的特点。旋转细胞培养系统的三维细胞培养(农村信用社)重新建立许多因素的表达,绒毛外滋养细胞系。

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