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JoVE Journal
Neuroscience

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Revealing Neural Circuit Topography in Multi-Color
 

Revealing Neural Circuit Topography in Multi-Color

Article DOI: 10.3791/3371
November 14th, 2011

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Summary November 14th, 2011

We provide a practical guide for delivering tracers in vivo and use the spinocerebellar pathway as a model system to demonstrate essential steps for successful neuronal circuit analysis in mice. We describe in detail our versatile tracing protocol that exploits wheat germ agglutinin (WGA) conjugated to Alexa fluorophores.

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