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JoVE Journal Neuroscience
Revealing Neural Circuit Topography in Multi-Color
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Revealing Neural Circuit Topography in Multi-Color

Article DOI: 10.3791/3371 • 09:11 min • November 14th, 2011
November 14th, 2011 •
1, 1, 1, 1
1Dominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, Yeshiva University

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Summary
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We provide a practical guide for delivering tracers in vivo and use the spinocerebellar pathway as a model system to demonstrate essential steps for successful neuronal circuit analysis in mice. We describe in detail our versatile tracing protocol that exploits wheat germ agglutinin (WGA) conjugated to Alexa fluorophores.

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Neural Circuit Topography Multi-color Neural Circuits Functional Topographic Maps Visualizing Circuit Architecture Labeling Topographic Projections Cerebellum Spinocerebellar Mossy Fibers Wheat Germ Agglutinin (WGA) Alexa 555 Alexa 488 Tracing Neural Projections Retrograde Transport Anterograde Transport Tracers Preparation Spinocerebellar Tracing Surgery Three-dimensional Imaging Functional Maps Organization Connectivity
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