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Imaging Neuronal Responses in Slice Preparations of Vomeronasal Organ Expressing a Genetically Encoded Calcium Sensor
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Chapters
- 00:05Title
- 01:48Preparation of Vomeronasal Organ Slice
- 04:45Imaging Chamber Setup
- 06:58Time Lapse Imaging
- 08:35Data Analysis
- 09:53Mouse VNO Response to Urine
- 10:38Conclusion
The vomeronasal organ (VNO) detects intraspecies chemical signals that convey social and reproductive information. We have performed Ca2+ imaging experiments using transgenic mice expressing G-CaMP2 in VNO tissue. This approach allows us to analyze the complicated response patterns of the vomeronasal neurons to large numbers of pheromone stimuli.
Tags
ImagingNeuronal ResponsesSlice PreparationsVomeronasal OrganGenetically Encoded Calcium SensorChemosensory SignalsSocialSexualReproductive StatusSpeciesPheromonesPredatorsVomeronasal Sensory NeuronsSpecificitySensitivityG-protein Coupled ReceptorsV1RV2RFPRDetection Of Chemosensory CuesPheromone Information EncodingResponse ProfilesStimuliSpecific ReceptorsNeuroepithelia Of VNOVomer BonesSemi-blind Tubular StructureVomeronasal DuctNasal CavityDendritesLumen Part Of VNOPheromone Cues Contact With ReceptorsCell Bodies Of VSNs Form Pseudo-stratified Layers
