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Journal / Medicine / Skeletmuskulatur Køn Dimorphism fra Proteomics
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Skeletmuskulatur Køn Dimorphism fra Proteomics

Article DOI: 10.3791/3536-v • 09:30 min • December 14th, 2011
December 14th, 2011 •
1, 2, 3, 4
1Center for Proteomics, Smith College, 2Department of Molecular Biophysics and Biochemistry, Yale University, 3Department of Chemistry, Smith College, 4Department of Biological Sciences and Center for Proteomics, Smith College

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En straight-forward sæt af metoder til at isolere og bestemme identiteten af ​​de hyppigst forekommende proteiner udtrykt i skeletmuskulatur. Ca. 800 spots skimtes på en to-dimensional gel fra 10 mg muskler, hvilket giver mulighed for bestemmelse af kønsspecifikke protein udtryk. Disse metoder vil give tilsvarende resultater i de fleste væv.

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Medicin skeletmuskulaturen køn 2-D gel elektroforese HPLC / MS mus
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