Biology
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In vivo Electroporation of Morpholinos into the Regenerating Adult Zebrafish Tail Fin
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Summary March 29th, 2012
We describe a method to conditionally knockdown the expression of a target protein during adult zebrafish fin regeneration. This technique involves micro-injecting and electroporating antisense oligonucleotide morpholinos into fin tissue, which allows testing the protein’s role in various stages of fin regeneration, including wound healing, blastema formation, and regenerative outgrowth.
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