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Biology

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JoVE Journal Biology
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In vivo Electroporation of Morpholinos into the Regenerating Adult Zebrafish Tail Fin

Article DOI: 10.3791/3632
March 29th, 2012 •
1, 2, 3
1Department of Biological Sciences, Center for Zebrafish Research, University of Notre Dame, 2Department of Microbiology, Immunology, and Pathology, Colorado State University, 3Departments of Anatomy and Cell Biology and Ophthalmology, Wayne State University School of Medicine

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March 29th, 2012

We describe a method to conditionally knockdown the expression of a target protein during adult zebrafish fin regeneration. This technique involves micro-injecting and electroporating antisense oligonucleotide morpholinos into fin tissue, which allows testing the protein’s role in various stages of fin regeneration, including wound healing, blastema formation, and regenerative outgrowth.

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