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JoVE Journal
Biology

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In vivo Electroporation of Morpholinos into the Regenerating Adult Zebrafish Tail Fin
 

In vivo Electroporation of Morpholinos into the Regenerating Adult Zebrafish Tail Fin

Article doi: 10.3791/3632
March 29th, 2012

Summary March 29th, 2012

We describe a method to conditionally knockdown the expression of a target protein during adult zebrafish fin regeneration. This technique involves micro-injecting and electroporating antisense oligonucleotide morpholinos into fin tissue, which allows testing the protein’s role in various stages of fin regeneration, including wound healing, blastema formation, and regenerative outgrowth.

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