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Journal
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Biology
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准备个人
果蝇
蛋钱伯斯实时成像
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Preparing Individual
Drosophila
Egg Chambers for Live Imaging
Please note that all translations are automatically generated.
Click here for the English version.
准备个人
果蝇
蛋钱伯斯实时成像
DOI:
10.3791/3679-v
•
10:38 min
•
February 27, 2012
•
Timothy T. Weil
,
Richard M. Parton
,
Ilan Davis
1
Department of Biochemistry
,
University of Oxford
Chapters
00:05
Title
01:22
Drosophila Ovary Dissection
02:30
Isolating Ovaries
04:32
Isolating Individual Late-stage Egg Chambers
05:38
Injection Preparation
09:28
Images of Dissected and Injected Oocytes
10:10
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
“<em>果蝇</em>蛋商会是一个很好的模型,为研究基因定位机制。为了捕捉支持本地化进程的动态事件,活组织快速高分辨率成像是必需的。在这里,我们提出了以最小的中断现场样品的解剖和成像协议。
Tags
Drosophila
Live Imaging
Preparation
Egg Chambers
Physiological Relevance
Imaging Conditions
Dehydration
Hypoxia
Overheating
Medium Deterioration
Body Patterning
MRNA Localization
Cytoskeletal Organization
Halocarbon Oil
Fluorescence Proteins
Transgenes
Labeled RNA
Protein Antibodies
MS2 Constructs
Genome
Oocyte Extraction
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