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August 08, 2012
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The chromium release assay, a common assay for detecting cytotoxic T cell activity, has several limitations. Using antigen-specific CD8 T cells and the human breast cancer tumor line, SKBR3, in the present article, an impedance-based approach was examined for the capability of detecting cell killing.
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Cite this Article
Erskine, C. L., Henle, A. M., Knutson, K. L. Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System. J. Vis. Exp. (66), e3683, doi:10.3791/3683 (2012).
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