Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / Spatio-Temporal Manipulation of Small GTPase…
JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content.

JoVE Journal Bioengineering
Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells
Experimental Quantification of Interactions Between Drug Delivery Systems and Cells In Vitro: A Guide for Preclinical Nanomedicine Evaluation
Journal (Bioengineering)
Experimental Quantification of Interactions Between Drug Delivery Systems and Cells In Vitro: A Guide for Preclinical Nanomedicine Evaluation
Tissue Engineering of the Intestine in a Murine Model
Journal (Bioengineering)
Tissue Engineering of the Intestine in a Murine Model
 

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells

Article DOI: 10.3791/3794-v • 10:28 min • March 9th, 2012
March 9th, 2012 •
1, 1, 1,2, 1,2, 2, 1,3, 1
1Department of Cell Biology, Center for Cell Dynamics, Johns Hopkins University, 2Graduate School of Pharmaceutical Sciences, University of Tokyo, 3Biomedical Engineering, Johns Hopkins University

Chapters

Summary
Automatic Translation

A method for spatio-temporal control of small GTPase activity by light is described. This method is based on rapamycin-induced FKBP-FRB heterodimerization and photo-caging systems. Optimization of light-irradiation enables the spatio-temporally controlled activation of small GTPases at the subcellular level.

Tags

Spatio-temporal Manipulation Small GTPase Activity Subcellular Level Seconds Living Cells Dynamic Regulation Rho Family Guanosine Triphosphatases Cellular Functions Visualization Real Time Perturbation Protein Activities Subcellular Location Timing Light-induced Activation Rapamycin-induced FKBP-FRB Heterodimerization Photo-caging Method Rapamycin Rapid Inducible Activation Inactivation Translocation Plasma Membrane FRB Anchor Photo-caging System Rapamycin Analogs
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter