A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation

Mariko Kobayashi; Ju-Youn Kim; Vladimir Camarena; Pamela C. Roehm; Moses V. Chao; Angus C. Wilson; Ian Mohr

doi:10.3791/3823

Journal

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Immunology and Infection

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A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation

17,213 Views

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12:22 min

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April 02, 2012

DOI:

10.3791/3823-v

DOI:

10.3791/3823-v

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12:22 min

April 02, 2012

•

5 Views

Mariko Kobayashi¹, Ju-Youn Kim¹, Vladimir Camarena², Pamela C. Roehm³, Moses V. Chao^2,4,5,6,7, Angus C. Wilson¹, Ian Mohr¹

¹Department of Microbiology,New York University School of Medicine, ²Molecular Neurobiology Program, Skirball Institute for Biomolecular Medicine,New York University School of Medicine, ³Department of Otolaryngology,New York University School of Medicine, ⁴Department of Cell Biology,New York University School of Medicine, ⁵Department of Physiology and Neuroscience,New York University School of Medicine, ⁶Department of Psychiatry,New York University School of Medicine, ⁷Center for Neural Science,New York University School of Medicine

Summary

The protocol describes an efficient and reproducible model system to study herpes simplex virus type 1 (HSV-1) latency and reactivation. The assay employs homogenous sympathetic neuron cultures and allows for the molecular dissection of virus-neuron interactions using a variety of tools including RNA interference and expression of recombinant proteins.