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JoVE Journal
Immunology and Infection

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A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation
 

A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation

Article DOI: 10.3791/3823
April 2nd, 2012

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Summary April 2nd, 2012

The protocol describes an efficient and reproducible model system to study herpes simplex virus type 1 (HSV-1) latency and reactivation. The assay employs homogenous sympathetic neuron cultures and allows for the molecular dissection of virus-neuron interactions using a variety of tools including RNA interference and expression of recombinant proteins.

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