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Chapters
- 00:05Title
- 01:55Preparing the STLV Bioreactor
- 04:01Preparing Microcarrier Beads
- 04:35Seeding Epithelial Cells and Beads into the STLV Bioreactor
- 06:45Culturing, Sampling, and Photo Documentation of Cultures
- 08:19Transferring and Harvesting Aggregates
- 09:273D-aggregates and Biology of Human Vaginal Epithelial Cells
- 11:59Conclusion
En roterende cellekultur system som gjør at epitelceller til å vokse under fysiologiske forhold som resulterer i 3-D mobilnettet aggregatdannelse er beskrevet. Aggregatene generert skjerm<em> In vivo</em>-Lignende egenskaper ikke observert i konvensjonelle kultur modeller og tjene som en mer nøyaktig organotypic modell for en rekke vitenskapelige undersøkelser.
Tags
Rotating Wall Vessel Bioreactor3D Epithelial Cell ModelsIn Vitro Cell CultureGrowth EnvironmentConventional Cell Culture SystemsPhysiologically Relevant MicroenvironmentRotating Wall Vessel (RWV) Bioreactor TechnologyOrganotypic ModelsExternal StimuliSuspension Culture SystemLow Physiological Fluid Shear ConditionsHigh-aspect Rotating Vessel (HARV)Slow-turning Lateral Vessel (STLV)Aeration Source
