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Chapters
- 00:05Title
- 01:55Preparing the STLV Bioreactor
- 04:01Preparing Microcarrier Beads
- 04:35Seeding Epithelial Cells and Beads into the STLV Bioreactor
- 06:45Culturing, Sampling, and Photo Documentation of Cultures
- 08:19Transferring and Harvesting Aggregates
- 09:273D-aggregates and Biology of Human Vaginal Epithelial Cells
- 11:59Conclusion
En roterande cellkultursystem som tillåter epiteliala celler att växa under fysiologiska betingelser som resulterar i 3-D-cellulär aggregatbildning beskrivs. Aggregaten genererade display<em> In vivo</em>-Liknande egenskaper observeras inte i konventionella kultur modeller och fungera som en mer korrekt organotypisk modellsystem för en mängd vetenskapliga undersökningar.
Tags
Rotating Wall Vessel Bioreactor3D Epithelial Cell ModelsIn Vitro Cell CultureGrowth EnvironmentConventional Cell Culture SystemsPhysiologically Relevant MicroenvironmentRotating Wall Vessel (RWV) Bioreactor TechnologyOrganotypic ModelsExternal StimuliSuspension Culture SystemLow Physiological Fluid Shear ConditionsHigh-aspect Rotating Vessel (HARV)Slow-turning Lateral Vessel (STLV)Aeration Source
