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Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry
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Bioengineering
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JoVE Journal Bioengineering
Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry

DOI:
10.3791/3884-v

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18:07 min

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June 08, 2012

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, , , , , , , , ,
1Department of Veterinary Microbiology and Preventive Medicine,Iowa State University, 2Amnis Corporation, 3Department of Chemical and Biological Engineering,Iowa State University

Chapters

  • 00:05Title
  • 02:15Assay Preparation: Mammalian and Bacterial Cell Culture/Nanoparticle Suspension
  • 03:48Phagocytosis Assay
  • 07:14Sample Acquisition on the ImageStreamX
  • 09:52Image Analysis
  • 15:33Representative Assay Results
  • 17:18Conclusion

Summary

Automatic Translation

Automatic Translation

In this article, we describe a method utilizing multi-spectral imaging flow cytometry to quantify the internalization of polyanhydride nanoparticles or bacteria by RAW 264.7 cells.

Tags

Cellular InternalizationNanoparticlesBacteriaMulti-spectral Imaging Flow CytometryVaccine DeliveryAntigen Presenting CellsNanoparticle FormulationHigh ThroughputQuantitative Experimental ProtocolMicroscopyFlow CytometryPhagocytosisStatistical DataSpatial ResolutionMulti-color Spectral FluorescenceBright Field Imaging

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