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Chapters
- 00:00Title
- 00:29Materials and Equipment
- 02:12Identifying Wells With Crystals
- 03:13Opening the Well
- 04:04“Closeup”: Opening Wells with Cubic Phase – Method I
- 06:38“Closeup”: Opening Wells with Cubic Phase – Method 2
- 08:27Opening Well with Sponge Phase – Unsuccessfully
- 10:25Opening Well with Sponge Phase – Successfully
- 14:20Harvesting Crystals
- 15:16Harvesting Crystals from the Cubic Phase
- 16:12Harvesting Crystals from the Sponge Phase
- 16:40Cryo-Cooling Crystals
- 17:25Storage/Transport Dewars
- 17:42Crystal Images
- 18:03Concluding Remarks
Herein is described procedures implemented in the Caffrey Membrane Structural and Functional Biology Group to harvest and cryo-cool membrane protein crystals grown in lipidic cubic and sponge phases for use in structure determination using macromolecular X-ray crystallography.
Tags
HarvestingCryo-coolingCrystalsMembrane ProteinsLipidic MesophasesStructure DeterminationMacromolecular CrystallographyIntegral Membrane ProteinsMacromolecular X-ray CrystallographyMX Diffraction Quality CrystalsG Protein-coupled Receptor FieldIn Meso MethodLipidic Cubic Phase MethodTechnical ChallengesViscous NatureSticky NatureMicro-crystalsManipulating CrystalsGlass Sandwich Plates
