Cryo-cooling Procedure
Cryo-cooling Procedure - Related Videos
18:45
Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography
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Cited by 44 •
2012
Herein is described procedures implemented in the Caffrey Membrane Structural and Functional Biology Group to harvest and cryo-cool membrane protein crystals grown in lipidic cubic and sponge phases for use in structure determination using macromolecular X-ray crystallography.
02:44
Heating and Cooling Curves
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2020
When a substance—isolated from its environment—is subjected to heat changes, corresponding changes in temperature and phase of the substance is observed; this is graphically represented by heating and cooling curves. For instance, the addition of heat raises the temperature of a solid; the amount of heat absorbed depends on the heat capacity of the solid (q = mcsolidΔT). According to thermochemistry, the relation between the amount of heat absorbed or released by a substance, q, and its...
04:58
Reduced Procedure Time and Variability with Active Esophageal Cooling During Radiofrequency Ablation for Atrial Fibrillation
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Cited by 4 •
2022
This study utilized advanced informatics techniques to compare procedure duration in patients undergoing radiofrequency atrial ablation treated with active esophageal cooling to those treated with traditional luminal esophageal temperature monitoring. Contextual inquiry, workflow analysis, and data mapping were utilized. The findings demonstrated reduced procedure time and variability with active cooling.
10:54
Cryo-electron Microscopy Specimen Preparation By Means Of a Focused Ion Beam
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Cited by 5 •
2014
Cryo Electron Microscopes, either Scanning (SEM) or Transmission (TEM), are widely used for characterization of biological samples or other materials with a high water content1. A SEM/Focused Ion Beam (FIB) is used to identify features of interest in samples and extract a thin, electron-transparent lamella for transfer to a cryo-TEM.
05:05
Preparation of High-Temperature Sample Grids for Cryo-EM
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Cited by 5 •
2021
This paper provides a detailed protocol for preparing sample grids at temperatures as high as 70 °C, prior to plunge freezing for cryo-EM experiments.
FAQs
Cryoprotective agents help limit damaging ice-crystal formation by reducing water movement as the sample cools. Their use supports preservation of biological structure and molecular state, which is especially important when cells, tissues, proteins, or organelles must remain suitable for later analysis. The choice to include them depends on the preservation strategy and the sample’s requirements.
Controlled cooling lowers temperature under managed conditions, helping reduce harmful ice formation while preserving sample organization. Rapid freezing instead aims to immobilize cellular components in a glass-like, vitrified state. These approaches serve related but distinct purposes: controlled cooling emphasizes gradual management of the freezing process, whereas rapid freezing focuses on trapping the sample in a noncrystalline state.
Cooling conditions influence how water moves and whether damaging ice crystals form. If the process is carefully controlled, biological structures and molecular states are more likely to remain close to their native organization. This preservation can improve imaging quality and protect specimens intended for storage or later investigation, making temperature control an important determinant of experimental outcomes.
A basic workflow must coordinate the sample, the cooling approach, and the intended preservation outcome. Researchers may use controlled cooling with cryoprotective agents or apply rapid freezing when vitrification is the goal. Maintaining the selected conditions throughout preparation and preservation helps retain structure and molecular state for subsequent analysis or storage.
Researchers choose cryo-cooling when they need to preserve biological material for analysis, storage, or both. In biology, the procedure supports cryo-electron microscopy, tissue and cell banking, and investigations of proteins, organelles, and whole cells. It is particularly useful when maintaining native organization matters for interpreting images or examining molecular and cellular structure.
For cryo-electron microscopy, preserving native organization can improve the quality and interpretability of images. For tissue and cell banking, limiting structural and molecular damage helps protect specimens for later use. The same preservation principles also support studies of proteins and organelles, allowing biological samples to remain available for investigation after cooling and storage.