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Produktion af lentivirusvektorer til transduktion af celler fra det centrale nervesystem
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System
DOI:

08:46 min

May 24, 2012

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Chapters

  • 00:05Title
  • 01:24Packaging of Lentiviral Vectors
  • 03:28Concentration and Purification of the Vectors
  • 04:50Titration of the Vectors
  • 06:25Transduction of Neocortical Cultures
  • 07:20Transduction Results
  • 08:25Conclusion

Summary

Automatic Translation

I denne protokol beskriver vi fremstilling, oprensning og titrering af lentivirale vektorer. Vi giver et eksempel lentivirale vektor-formidlet gen-levering i primære dyrkede neuroner og astrocytter. Vores metoder kan også gælde for andre celletyper<em> In vitro</em> Og<em> In vivo</em>.

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