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JoVE Journal
Biology

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A Semi-quantitative Approach to Assess Biofilm Formation Using Wrinkled Colony Development
 

A Semi-quantitative Approach to Assess Biofilm Formation Using Wrinkled Colony Development

Article DOI: 10.3791/4035
June 7th, 2012

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Summary June 7th, 2012

We provide a simple, semi-quantitative method to investigate biofilm formation in vitro. This method takes advantage of the Zeiss stemi 2000-C Dissecting Microscope (with camera attachment) to monitor both the timing and pattern of biofilm formation, as assessed by the development of wrinkled colonies.

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