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The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions
 

The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions

Article doi: 10.3791/4072
December 15th, 2012

Summary December 15th, 2012

The Green Monster method enables the rapid assembly of multiple deletions marked with a reporter gene encoding green fluorescent protein. This method is based on driving yeast strains through repeated cycles of sexual assortment of deletions and fluorescence-based enrichment of cells carrying more deletions.

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