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The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions
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The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions

The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions

DOI:
10.3791/4072-v

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13:06 min

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December 15, 2012

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, , , , ,
1Department of Synthetic Biology and Bioenergy,J. Craig Venter Institute, 2Department of Microbial and Environmental Genomics,J. Craig Venter Institute, 3Donnelly Centre & Department of Molecular Genetics,University of Toronto, 4Lunenfeld Research Institute,Mt Sinai Hospital

Chapters

  • 00:05Title
  • 01:38Sexual Cycling
  • 06:13Flow Cytometry
  • 09:26Genotyping
  • 10:28Results: Yeast Strains Carrying Multiple Gene Deletions as Generated by the Green Monster Process
  • 12:47Conclusion

Summary

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The Green Monster method enables the rapid assembly of multiple deletions marked with a reporter gene encoding green fluorescent protein. This method is based on driving yeast strains through repeated cycles of sexual assortment of deletions and fluorescence-based enrichment of cells carrying more deletions.

Tags

Green Monster ProcessYeast StrainsGene DeletionsPhenotypesGenetic BackgroundEnvironmental ConditionMutation TestingHidden Gene FunctionsGenetic InteractionsMulti-mutant InteractionsParalogsSaccharomyces CerevisiaeGene KnockoutSelectable MarkerHomologous RecombinationMarker RemovalGreen Monster MethodGreen Fluorescent Protein (GFP) Reporter

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