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JoVE Journal
Biology

This content is Open Access.

High-throughput Purification of Affinity-tagged Recombinant Proteins
 

High-throughput Purification of Affinity-tagged Recombinant Proteins

Article DOI: 10.3791/4110
August 26th, 2012

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Summary August 26th, 2012

We describe a method for the affinity-tagged purification of recombinant proteins using liquid-handling robotics. This method is generally applicable to the small-scale purification of soluble His-tagged proteins in a high-throughput format.

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