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DNA Vector-based RNA Interference to Study Gene Function in Cancer
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JoVE Journal Medicine
DNA Vector-based RNA Interference to Study Gene Function in Cancer

DNA Vector-based RNA Interference to Study Gene Function in Cancer

English

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20,292 Views

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13:10 min

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June 04, 2012

DOI:

10.3791/4129-v

DOI:
10.3791/4129-v

•

13:10 min
June 04, 2012

•

4 Views
, , , ,
1Department of Cancer Biology and Comprehensive Cancer Center,Wake Forest University School of Medicine, 2Department of Pathology and Comprehensive Cancer Center,Wake Forest University School of Medicine

Summary

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RNA interference (RNAi) possesses many advantages over gene knockout and has been broadly used as a tool in gene functional studies. The invention of DNA vector-based RNAi technology has made long term and inducible gene knockdown possible, and also increased the feasibility of gene silencing in vivo.

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Cite this Article

Stovall, D. B., Wan, M., Zhang, Q., Dubey, P., Sui, G. DNA Vector-based RNA Interference to Study Gene Function in Cancer. J. Vis. Exp. (64), e4129, doi:10.3791/4129 (2012).

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