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Studieren Proteolyse von Cyclin B an der Single Cell Level in Whole Cell Populationen
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Studieren Proteolyse von Cyclin B an der Single Cell Level in Whole Cell Populationen

Article DOI: 10.3791/4239-v • 10:55 min • September 17th, 2012
September 17th, 2012 •
1, 1, 1, 1, 1
1Department of Hematology, Oncology and Stem Cell Transplantation, University Medical Center Freiburg

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Metaphase zur Anaphase Übergang durch Anaphase-promoting complex (APC / C)-abhängige Ubiquitinierung und anschließende Zerstörung von Cyclin B. Hier haben wir ein System, das Pulse-Chase-Kennzeichnung nach, ermöglicht die Überwachung Cyclin B Proteolyse im gesamten Zellpopulationen und wird ausgelöst, erleichtert den Nachweis von Störungen durch den mitotischen Checkpoint.

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Genetik Zellbiologie Molekularbiologie Proteomics Cyclin B Spindel Checkpoint Anaphase-promoting complex Mitose Proteasom-abhängigen Proteolyse SNAP Zellzyklus
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