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Ex vivo Culturing of Whole, Developing Drosophila Brains

Article DOI: 10.3791/4270 • 08:39 min • July 27th, 2012
July 27th, 2012 •
1,2, 1,2, 1,2
1National Institute of Neurological Disorders and Stroke, 2National Human Genome Research Institute, National Institutes of Health, Bethesda, MD

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Summary
Automatic Translation

This article describes a method by which one can mimic in vivo development of the Drosophila mushroom body in an ex vivo culture system.

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Ex Vivo Culturing Drosophila Brains Chronic Genetic Manipulations Cell Biology Development Central Brain Structures Pharmacological Interventions Live Imaging Subcellular Compartment Axon Initial Segment (AIS) Neuron-specific Cyclin-dependent Kinase Cdk5 Pharmacological Inhibitors Actin Organization Cell-surface Protein Fasciclin 2 Mutants Remodeling Connections Embryonic Mushroom Body (MB) Metamorphosis Larva To Adult Olfactory Learning And Memory
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