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Subretinal injektion af genterapivektorer og stamceller i mus Eye
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JoVE Journal Medicine
Subretinal Injection of Gene Therapy Vectors and Stem Cells in the Perinatal Mouse Eye

Subretinal injektion af genterapivektorer og stamceller i mus Eye

DOI:
10.3791/4286-v

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05:09 min

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November 25, 2012

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, , , ,
1Bernard and Shirlee Brown Glaucoma Laboratory, Department of Ophthalmology,Columbia University , 2Institute of Human Nutrition, College of Physicians & Surgeons,Columbia University , 3Omics Laboratory,University of Iowa , 4Department of Ophthalmology and Visual Sciences,University of Iowa

Chapters

  • 00:00Title
  • 00:09Injection Device Assembly
  • 01:04Aliquot Vector or Cells
  • 01:28Subretinal Injection
  • 02:00Sclerotomy
  • 02:54Subretinal Injection
  • 04:51Summary

Summary

Automatic Translation

Automatic Translation

Denne kirurgiske teknik illustrerer injektion af genterapivektorer og stamceller i det subretinale rum af muse øjet.

Tags

Subretinal InjectionGene Therapy VectorsStem CellsPerinatal Mouse EyeBlindnessRetinal Degenerative DiseasesAutologous TransplantationAge-related Macular Degeneration (AMD)Iris Pigment Epithelial Cell TransplantationRPE65 Gene Replacement TherapyLeber’s Congenital AmaurosisRPE Cell TransplantationStargardt’s DiseaseAnimal ModelsTherapeutic Efficacy

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