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JoVE Journal
Biology

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Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle
 

Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle

Article DOI: 10.3791/50038
March 31st, 2013

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Summary March 31st, 2013

Cilia-generated fluid flow in Kupffer’s Vesicle (KV) controls left-right patterning of the zebrafish embryo. Here, we describe a technique to modulate gene function specifically in KV cells. In addition, we show how to deliver fluorescent beads into KV to visualize fluid flow.

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