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Journal / Neuroscience / Generering av Lokalt Transgena råttor genom
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Neuroscience

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JoVE Journal Neuroscience
Generering av Lokalt Transgena råttor genom
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Generering av Lokalt Transgena råttor genom

Article DOI: 10.3791/50146-v • 08:44 min • September 24th, 2013
September 24th, 2013 •
1, 2, 2, 3, 1
1Department of Neuropathology, Medical School Düsseldorf, 2Department of Molecular Genetics, Weizmann Institute for Science, 3Center of Behavioral Neuroscience, University of Düsseldorf

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Gener kan manipuleras under utvecklingen av cortex eller hippocampus hos råtta genom i livmodern elektroporering (IUE) på E16, för att möjliggöra snabba och målinriktade ändringar i nervkopplingar för senare studier av beteende eller neuropatologi hos vuxna djur. Förlossnings in vivo imaging för kontroll av IUE framgång utförs av mareld att aktivera samtransfekterad luciferas.

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Neurovetenskap Hippocampus Minne schizofreni, luciferas Störd-in-schizofreni-1 (DISC1)
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