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The Use of Reverse Phase Protein Arrays (RPPA) to Explore Protein Expression Variation within Individual Renal Cell Cancers
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The Use of Reverse Phase Protein Arrays (RPPA) to Explore Protein Expression Variation within Individual Renal Cell Cancers

The Use of Reverse Phase Protein Arrays (RPPA) to Explore Protein Expression Variation within Individual Renal Cell Cancers

DOI:
10.3791/50221-v

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12:22 min

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January 22, 2013

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, , , , , , , , , , ,
1Edinburgh Urological Cancer Group,University of Edinburgh, 2School of Medicine,University of St Andrews, 3Division of Pathology,University of Edinburgh, 4MRC Human Genetics Unit, MRC IGMM,University of Edinburgh, 5Department of Pathology,Western General Hospital, 6Breakthrough Breast Cancer Research Unit,University of Edinburgh, 7St Bartholomew’s Cancer Institute, Experimental Cancer Medicine Centre,Queen Mary University of London

Chapters

  • 00:05Title
  • 01:39Identification of Morphological and Molecular Tumor Heterogeneity
  • 02:58Protein Extraction from Tumor Samples
  • 04:24RPPA Printing
  • 06:00RPPA Protein Detection
  • 09:27Results: Pre- and Post-TKI-treated Renal Cell Cancer Show Differential Protein Expression
  • 12:06Conclusion

Summary

Automatic Translation

Automatic Translation

RPPA enables the protein expression of hundreds of samples, printed on nitrocellulose slides to be interrogated simultaneously, using fluorescently labelled antibodies. This technique has been applied to study the effect of drug treatment heterogeneity within clear cell renal carcinoma.

Tags

Keywords: Reverse Phase Protein Arrays (RPPA)Renal Cell CancerProtein ExpressionTumor HeterogeneityTargeted TherapyTyrosine Kinase InhibitorPersonalized TreatmentAntibody ValidationProteomic Analysis

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