Biotinylated Primer
Biotinylated Primer - Related Videos
09:13
Cell-surface Biotinylation Assay
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2023
A cell can regulate the amount of particular proteins on its cell membrane through endocytosis, following which cell surface proteins are effectively sequestered in the cytoplasm. Once within a cell, these surface proteins can be either destroyed or “recycled” back to the membrane. The cell surface biotinylation assay provides researchers with a way to study these phenomena. The technique makes use of a derivative of the small molecule biotin, which can label surface proteins and then be...
11:14
Primer-Free Aptamer Selection Using A Random DNA Library
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Cited by 20 •
2010
SELEX protocols comprise multiple rounds of selection, each of which require regeneration of bound ligands, which in turn require fixed primer sequences flanking the random library regions. These fixed primer sequences can interfere with the selection process (false positives and negatives). Here we present a primer-free protocol.
11:32
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
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Cited by 20 •
2017
Here, we detail the method of Sequencing of Psoralen crosslinked, Ligated, and Selected Hybrids (SPLASH), which enables genome-wide mapping of intramolecular and intermolecular RNA-RNA interactions in vivo. SPLASH can be applied to study RNA interactomes of organisms including yeast, bacteria and humans.
06:43
In Vivo Proximity Biotinylation for Protein Interaction Studies in Paramecium tetraurelia
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Cited by 1 •
2025
The protocol presents a method for in vivo covalent attachment of biotin to proteins based on their proximity to a biotin ligase fused to a protein of interest. This modification allows for a selective enrichment of the proteins using streptavidin beads as needed in protein interaction studies.
15:28
Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
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Cited by 41 •
2009
We present a method of targeted ancient DNA sequence retrieval, which we used to reconstruct the complete mitochondrial genomes of five Neandertal individuals. Comparison of these sequences with present day humans suggests that Neandertals had a long term low effective population size.
FAQs
The label is covalently attached to the primer before amplification begins. When the primer anneals to its complementary template, DNA polymerase extends that primer, so the biotin-containing primer becomes part of the newly generated product. This places biotin on the amplified material itself rather than requiring a separate labeling step after amplification.
Biotin binds strongly to streptavidin or avidin, creating a selective connection between the amplified nucleic acid and a capture reagent. If streptavidin is associated with magnetic beads or a surface, the product can be separated, immobilized, or presented for detection. The interaction therefore converts a molecular label into a practical handling and signal-generation tool.
Both primers direct template-specific amplification through annealing and polymerase extension, but the biotinylated version adds a controllable capture feature to the resulting product. An unlabeled product does not gain that biotin-dependent route for binding streptavidin or avidin. Consequently, labeling supports downstream purification, immobilization, hybridization, or detection beyond amplification itself.
First, the labeled primer is used in a primer-directed amplification reaction so the target product incorporates biotin. The amplified material is then exposed to streptavidin- or avidin-associated magnetic beads or another capture surface. Binding enables selective retention or immobilization, allowing the labeled product to be handled separately from material that lacks the biotin-linked interaction.
These products support several workflows that require selective nucleic acid handling. The overview identifies nucleic acid purification, hybridization assays, sequencing workflows, probe-based detection, and immobilization on surfaces as applications. In each case, the incorporated label provides a means to capture, retain, position, or detect amplified DNA or RNA through avidin or streptavidin binding.
After amplification, the biotin on the product can bind streptavidin or avidin linked to a capture surface, magnetic bead, probe-based system, or signal-generating component. Immobilizing the product in this way can support hybridization assays and detection workflows. The approach connects sequence-specific amplification with selective positioning and observation of the resulting nucleic acid.