Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

Journal

Biology

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

DOI:

10.3791/50589-v

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09:20 min

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September 02, 2013

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Jacek R. Wiśniewski

¹Department of Proteomics and Signal Transduction,Max Planck Institute of Biochemistry

Chapters

00:05Title
01:46Preparation of Tissue Slices for Microdissection and Sample Lysis
03:20Sample Processing
04:51Quantification of the Peptides
05:38Fractionation of the Lys-C and Tryptic Peptides
07:29Results: Peptide Quantification
08:21Conclusion

Summary

Automatic Translation

Archival formalin fixed and paraffin embedded (FFPE) clinical samples are valuable material for investigation of diseases. Here we demonstrate a sample preparation workflow allowing in-depth proteomic analysis of microdissected FFPE tissue.

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

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