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Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

Article DOI: 10.3791/50589 • 09:20 min • September 2nd, 2013
September 2nd, 2013 •
1
1Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry

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Archival formalin fixed and paraffin embedded (FFPE) clinical samples are valuable material for investigation of diseases. Here we demonstrate a sample preparation workflow allowing in-depth proteomic analysis of microdissected FFPE tissue.

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Proteomic Sample Preparation Formalin-fixed And Paraffin-embedded Tissue Clinical Material Quantitative Analysis FFPE Material Microdissection Filter Aided Sample Preparation FASP Technique Endoproteinase LysC Trypsin Digestion Fluorescence Measurement Peptide Fractionation Proteomes Diseases Biomarkers Drug Targets
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