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JoVE Journal
Immunology and Infection

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Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells
 

Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells

Article DOI: 10.3791/50729
September 5th, 2013

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Summary September 5th, 2013

Central to the field of bacterial pathogenesis is the ability to define if and how microbes survive after exposure to eukaryotic cells. This article outlines protocols for the use of fluorescent dyes that reveal the viability of individual bacteria inside and associated with host cells.

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